The invention discloses an application of dipeptide diaminobutyroyl benzylamide diacetate in the preparation of a skin ulcer treatment medicine. The skin ulcer prevention or treatment medicine includes dipeptide diaminobutyroyl benzylamide diacetate and pharmaceutically acceptable auxiliary materials, and every 1000 g of the medicine contains 0.1-5.0 mg of the dipeptide diaminobutyroyl benzylamide diacetate; and the dosage of the medicine is a solution, an ointment, a gel, a microsphere or a spray. The medicine has a good skin ulcer treatment effect, and the adding sequence and the addition amount of the medicine component in the medicine are strictly controlled to make the dipeptide diaminobutyroyl benzylamide diacetate be well dissolved in a solvent and perform synergistic effects together with the auxiliary materials.