The present invention provides a preparation method of a glass surface with a partial frosted area. The glass surface comprises a frosted area and a non-frosted area, wherein the dividing line between the frosted area and the non-frosted area is a straight line. The preparation method comprises: carrying out screen printing of protection ink, wherein the non-sand area is subjected to two-layer acid-resistant ink overlapping screen printing, and the total thickness of the two layers of the acid-resistant ink is 8-20 [mu]m; sandblasting, wherein the frosted area of the glass is sandblasted by using a sandblasting machine with 4-16 spraying nozzles, the glass is pushed to move forwardly along with a conveying belt during the sandblasting, and the sand is angular alumina and/or silicon carbide; and carrying out acid washing, and carrying out fade plating of the protection ink. According to the present invention, the screen printing of the ink protection is set before the sandblasting, and the specific operation modes and the parameters of the sandblasting and the acid washing are controlled, such that the step position deviation of the glass surface with the partial frosted area is less than 20 [mu]m, and the linearity of the sandblasting dividing line is less than 30 [mu]m.