The invention relates to a makeup remover composition and a preparation method and application thereof. The makeup remover composition is composed of a surfactant and polyol, wherein the surfactant is a non-ionic surfactant including an ethoxylated glyceride and at least one ethylene oxide-epoxypropane block copolymer, and the polyol is one or more selected from the group consisting of glycerin, propylene glycol, polyethylene glycol and butylene glycol. Cleansing water prepared from the makeup remover composition has the advantages of no irritation, no need for cleaning and refreshing performance.