The invention relates to the field of a fuel cell, in particular to a direct methanol fuel cell anode catalyst and a preparation method thereof. The anode catalyst is formed by loading RuNi nanometer alloy on a surface of C-coated porous nanometer hollow TiO2 (C@TiO2) to obtain RuNi/C@TiO2 and then coating the RuNi/C@TiO2 with high-conductivity porous spongy polymer polyaniline-poly(2-acrylamide-2-methyl propane sulfonic acid), wherein the mass content of the C@TiO2 accounts for 97-99% of the RuNi/C@TiO2, the mass content sum of the RuNi nanometer alloy accounts for 3-1% of the RuNi/C@TiO2, the mole ratio (nRu:nNi) of Ru and Ni in the RuNi alloy is (7:3)-(3:7), the mole ratio of aniline and 2-acrylamide-2-methyl propane sulfonic acid in the polymer is 2:1, and the mole ratio of the aniline and the TiO2 is (3-1):1. The preparation method of the anode catalyst is also described in detail. By the anode catalyst, the cost of the direct methanol fuel cell catalyst can be reduced, the stability of the direct methanol fuel cell catalyst is improved, and the catalytic activity and the CO poison-resistant capability on methanol are improved.