The invention discloses a warp re-beaming machine. The warp re-beaming machine comprises a machine base and a machine head. A plurality of supports are arranged on the machine base, loom beams are arranged at the top of the support, a warp beam is arranged on the machine head, a yarn separating mechanism is arranged at the portion, between the loom beams and the warp beam, of the machine head, and comprises a yarn separating frame, a through hole is formed in the top of the yarn separating frame, a plurality of needle frames are arranged on the yarn separating frame, a plurality of isolation needles are vertically fixed on the needle frames, the adjacent needle frames are sequentially hinged, sliding blocks rotationally connected with the needle frames are arranged below the middles of the needle frames, a pair of threaded holes with the reverse screw directions are formed in the sliding blocks located at the two ends of the yarn separating frame, a threaded rod is matched with the threaded holes, supporting frames are arranged at the two ends of the yarn separating frame, the supporting frames and the threaded rod are axially fixed and circumferentially rotate, and a driving mechanism is arranged at the bottom of the yarn separating frame and fixed on the machine head. According to the warp re-beaming machine, the service life is prolonged, movable adjustment of the isolation needles on the yarn separating mechanism is controlled, and therefore the isolation needles with the suitable quantity can be selected and used as required.