The invention relates to a click chemistry-based cotton fabric durable anti-crease finishing method. The click chemistry-based cotton fabric durable anti-crease finishing method comprises the following steps: putting coarsened cotton fabric into a 3-thiopropyl triethoxy silane solution and performing sulfydryl modification to obtain sulfydryl modified cotton fabric; ultrasonically mixing photocurable waterborne polyurethane acrylate, sulfydryl silane and a photoinitiator to obtain finishing liquid; dipping the sulfydryl modified cotton fabric into the finishing liquid and performing ultraviolet curing to obtain the durable anti-crease cotton fabric. The method provided by the invention is simple in operation, high in efficiency, energy-saving and environmentally friendly; after finishing is conducted, the anti-crease effect is remarkable, the durability is high, and the influence on the whiteness of the fabric is little. After the cotton fabric is finished by the anti-crease process, the wearability can be improved, the application field can be enlarged, and the requirements of people on flat appearance and wear comfortability of clothes can be met.