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发明授权
CN107254476B 绿豆抗豆象基因VrPGIP等位基因VrPGIP1-2及其应用 失效 - 权利终止

绿豆抗豆象基因VrPGIP等位基因VrPGIP1-2及其应用
摘要：
本发明公开了绿豆抗豆象基因VrPGIP等位基因VrPGIP1‑2及其应用。通过对绿豆抗豆象品种V2709的候选基因VrPGIP进行克隆和测序，与感豆象绿豆品种的VrPGIP序列进行比对，结果表明，V2709在VrPGIP的554bp处发生单碱基替换，由C变为G，这一碱基突变导致第185个氨基酸由脯氨酸变为精氨酸，从而引起VrPGIP蛋白功能的变化。这与已鉴定的VrPGIP基因的突变位置不同，表明这是VrPGIP的一个新等位基因，命名为VrPGIP1‑2。本发明还成功开发出VrPGIP1‑2的功能性分子标记，对绿豆抗豆象育种工作具有重要意义。
摘要（英）：

The invention discloses an allele VrPGIP1-2 of a bruchid resistance gene VrPGIP of a mung bean and application of the allele VrPGIP1-2 of the bruchid resistance gene VrPGIP of the mung bean. The candidate gene VrPGIP of a bruchid resistance variety V2709 of the mung bean is cloned and sequenced, the sequence of the candidate gene VrPGIP and the sequence of a bruchid resistance variety VrPGIP of the mung bean are compared, the result shows that the V2709 is subjected to single-base replacement on 554bp of the VrPGIP, C is changed to G, and the base mutation leads to transformation of proline to arginine on the 185th amino acid, so that the protein function of the VrPGIP is changed. The location is different from the mutation location of the identified gene VrPGIP, thereby showing that the VrPGIP is a new allele named VrPGIP1-2. The invention further successfully develops a functional molecular marker VrPGIP1-2 which is of great significance for the breeding of bruchid resistance of the mung bean.

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