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发明公开
CN107201335A 一种Marc‑145细胞培养液及其制备方法、使用方法 无效 - 驳回

一种Marc‑145细胞培养液及其制备方法、使用方法
摘要：
一种Marc‑145细胞培养液及其制备方法、使用方法，属于兽用生物制品技术领域。该细胞培养液每1000ml去离子水中含有以下组分：DMEM粉末、胰岛素生长因子、甘氨酸锌、亚硒酸钠、碳酸氢钠、转铁蛋白、人参皂苷、淫羊藿多糖。本发明具有以下有益效果：本发明所使用的Marc‑145细胞培养液，大大减少了血清的使用量，降低了细胞培养的成本；提高Marc‑145细胞生长速度；增强PRRSV对Marc‑145细胞的敏感性，提高了病毒含量；由本发明所使用的Marc‑145细胞培养液还可以大幅度提高猪繁殖与呼吸综合征疫苗的免疫原性。
摘要（英）：

The invention provides a Marc-145 cell culture liquid, and a preparation method and a use method thereof, and belongs to the technical field of biological products for veterinary use. In the cell culture liquid, per 1000 ml of deionized water contains the following components: a DMEM powder, insulin-like growth factors, zinc glycinate, sodium selenite, sodium bicarbonate, transferring, ginsenoside and epimedium polysaccharide. The Marc-145 cell culture liquid, and the preparation method and the use method thereof have the following beneficial effects: the Marc-145 cell culture liquid greatly reduces the use amount of serum and reduces the cell culture cost; the growth speed of the Marc-145 cells is increased; the sensitivity of PRRSV (porcine reproductive and respiratory syndrome) on the Marc-145 cells is enhanced and the virus content is increased; the Marc-145 cell culture liquid can greatly improve the immunogenicity of PRRSV vaccines.

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