The invention relates to a method for flotation separation of copper pyrites and arsenopyrite in copper rough concentrate, and belongs to the technical field of mineral processing. The mass percent concentration and pH of pulp of the copper rough concentrate are regulated, Na2S2O3, NH4Cl and CuSO4 are sequentially added to conduct conventional flotation to obtain a rough concentrate product, and the rough concentrate product is subjected to multiple times of concentration to obtain a final rough concentrate product. The method effectively eliminates the activation effect of copper ions to arsenopyrite, an AsO4NH4Cu-6H2O hydrophilic film generated after reaction prevent xanthic acid from being adsorbed on the surface of arsenopyrite. Meanwhile, a copper sulfide film is generated on the surface of copper pyrites, adsorption of xanthic acid on the surface of the copper pyrites is promoted, the hydrophobicity of the copper pyrites is improved, and thus the floatability difference between the copper pyrites and the arsenopyrite is improved.