The invention belongs to the field of food sanitation and safety rapid detection, and provides a primer group having specificity to specific gene segments of escherichia coli O157:H7, staphylococcus aureus, salmonella, listeria monocytogenes and vibrio parahaemolyticus, a micro-fluidic chip on which the primer group is fixed, and a kit including amplification reaction liquid to detect the escherichia coli O157:H7, the staphylococcus aureus, the salmonella, the listeria monocytogenes and the vibrio parahaemolyticus. A micro-fluidic chip rapid detection technology for five food-borne pathogens and the kit provided by the scheme have the advantages of high sensitivity, high specificity, convenience, rapidness, wide application range and the like, and problems concerned with on-site rapid detection and basic-level popularization and application of the food-borne pathogens can be solved; particularly, the technology and the kit can be applied to on-site detection and wartime wild places. Meanwhile, the kit has the advantages of low reagent consumption, low pollution, low cost, convenience in carrying and the like.