The invention discloses an automobile wire harness detection device and a use method. The automobile wire harness detection device comprises a box body and a standard wire group. The box body is connected with a handle in a hinged manner. The front surface of the box body is provided with a detection port I, a detection port II, a display device, and a contact selection button. The contact selection button is provided with eleven shifts from zero to ten. A power supply device, a protection resistor, a current measuring device, and a voltage measuring device are disposed in the box body. The detection port I, the detection port II, the display device, the protection resistor, the current measuring device, and the voltage measuring device are electrically connected with the power supply device. The automobile wire harness detection device has advantages of simple structure, low costs, easy operation, portability, and thorough detection results.