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发明公开
CN107121549A 一种快速的检测癌胚抗原的比色分析方法 失效 - 权利终止

一种快速的检测癌胚抗原的比色分析方法
摘要：
本发明涉及医学中肿瘤标志物检测领域，具体是利用标记物构建比色免疫传感器，实现对癌胚抗原的检测。一种快速的检测癌胚抗原的比色分析方法，制备Ag3PO4/Ag纳米复合材料并将其修饰癌胚抗原抗体，制备Fe3O4/Ag纳米复合材料并将其修饰癌胚抗原抗体，将Fe3O4@Ag‑Ab1纳米球分散液依次孵育不同浓度的癌胚抗原CEA 30分钟，然后用水清洗2‑5次，再用Ab2‑Ag3PO4/Ag纳米球分散液孵育30分钟，构建成免疫传感器，加入ABS缓冲液和四甲基联苯胺TMB溶液，通过紫外分光光度计检测TMB的紫外吸收来定量CEA的浓度。
摘要（英）：

The invention relates to the field of medical detection of tumor markers. Particularly, a colorimetric immune sensor is constructed by using a marker to realize detection of a carcino-embryonic antigen. A colorimetric analysis method for quickly detecting carcino-embryonic antigen comprises the following steps: preparing a Ag3PO4/Ag nano composite material, modifying a carcino-embryonic antigen antibody with the Ag3PO4/Ag nano composite material, preparing a Fe3O4/Ag nano composite material, modifying the carcino-embryonic antigen antibody with the Fe3O4/Ag nano composite material, incubating carcino-embryonic antigen (CEA) with different concentrations through a Fe3O4@Ag-Ab1 nanosphere dispersion solution for 30 minutes, then cleaning with water for 2 to 5 times, incubating the CEA through Ab2-Ag3PO4/Ag nanosphere dispersion solution for 30 minutes, thus constructing an immune sensor, adding an ABS buffer solution and a tetramethyl benzidine (TMB) solution, and detecting ultraviolet absorption of TMB through an ultraviolet spectrophotometer to quantify the concentration of the CEA.

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