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发明公开
CN107119072A 一种过表达ZEB2基因质粒及其构建方法和应用 有权

一种过表达ZEB2基因质粒及其构建方法和应用
摘要：
本发明公开了一种过表达ZEB2基因质粒，由ZEB2基因CDS序列与pEGFP‑C2真核表达载体的重组构建而成；其核苷酸如序列表SEQ ID NO:1，位于ZEB2基因的第134‑3778位；氨基酸序列如序列表SEQ ID NO:2所示。本发明还公开了一种过表达ZEB2基因质粒的构建方法和应用。本发明的优点在于：(1)有助于研究ZEB2基因功能，并且为研究其对炎症在AKI病程中的作用提供了有用分子生物学工具；(2)为进一步研究ZEB2的功能，以及AKI的基因治疗奠定了基础。
摘要（英）：

The invention discloses an overexpression ZEB2 gene plasmid which is obtained by reconstructing a ZEB2 gene CDS and a pEGFP-C2 eukaryotic expression vector. A nucleotide sequence of the plasmid is shown in SEQ ID NO (sequence identifier number): 1 in a sequence table and located in the 134-3778 site of a ZEB2 gene, and an amino acid sequence of the plasmid is shown in SEQ ID NO: 2 in the sequence table. The invention further discloses a construction method and an application of the overexpression ZEB2 gene plasmid. The plasmid, the method and the application have the advantages that (1) research of a function of the ZEB2 gene is facilitated; a useful molecular biology tool is provided for researching an effect of the ZEB2 gene on inflammation in a course of an AKI (acute kidney injury), and (2) a foundation is laid for further research of the function of ZEB2 and gene therapy of the AKI.

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