The invention belongs to the technical field of paints, and particularly relates to water-based fruit and vegetable fresh-keeping paint and a preparation method thereof. According to the water-based fruit and vegetable fresh-keeping paint and the preparation method thereof, aqueous extract liquid of platycodon roots, aqueous extract liquid of tree peony bark, aqueous extract liquid of wild chrysanthemums, and aqueous extract liquid of rhizoma gastrodiae are used as preservatives, mixed liquid which is prepared by modifying a mixture consisting of D-calcium pantothenate, calcium glycerophosphate and zinc sulfate through a phosphate aqueous solution of which the pH is 4.3-5.2 is used as a fruit and vegetable softening inhibitor, an emulsifying agent, a thickening agent and a gelling agent are matched for use, so that the water-based fruit and vegetable fresh-keeping paint is prepared. The water-based fruit and vegetable fresh-keeping paint is simple in components, the used materials are green and non-toxic and the paint can be prepared without severe production equipment.