The invention discloses a multi-layer shaking screen. The multi-layer shaking screen comprises a cylinder body and a vibration motor; the bottom end of the cylinder body is fixedly connected with a machine base through strong springs, and nut bases are fixed at the edge of the upper end of the machine base and are rotationally connected with limiting bolts in the nut bases in a thread engagement manner; a feeding hole is formed in a dustproof upper cover at the top end of the cylinder body and is formed in the vertical axis of the dustproof upper cover; a first-stage filter net, a second-stage filter net and a third-stage filter net are sequentially installed inside the cylinder body from top to bottom, and certain gaps are formed between the second-stage filter screen and the first-stage filter screen as well as between the second-stage filter screen and the third-stage filter screen separately; and the vibration motor is fixedly connected with the inner wall at the lower end of the cylinder body through a mounting ring, and heavy hammers are installed on an upper rotating shaft and a lower rotating shaft of the vibrating motor. According to the multi-layer shaking screen, the first-stage filter net, the second-stage filter net and the third-stage filter net are installed inside the cylinder body and have the multistage filtering function, fine screening can be carried out on the materials, and different requirements of users can be met.