The invention discloses a fault detection method of a service function chain in the network function virtualization. The detection method comprises the following steps: a first end point S sends a fault detection request message to a downstream node thereof by use of T1, if the downstream node of the first end point S forwards the fault detection request message to the downstream node thereof after receiving the fault detection request message, an upstream node forwards the fault detection request message to the downstream node thereof until a second end point D receives the fault detection request message; the second end point D forwards a fault detection response message to the upstream node thereof after receiving the fault detection request message; the downstream node orderly forwards the fault detection response message to the upstream node thereof until the first end point S receives the fault detection response message; if the fault detection response message is not received in the T2, the existence of the fault is judged. By use of the fault detection method disclosed by the invention, the position in fault can be quickly positioned, the fault detection and positioning control message of the transmission in the network is reduced at the same time, and the cost is lowered.