The invention discloses an inoculation identification method for clubroot of cruciferous crops, belongs to the technical field of plant protection, and provides the inoculation identification method for clubroot of cruciferous crops. Aseptic dried sphagnum is used as a carrier for culturing Plasmodiophora by inoculating. The method of the invention has the advantages that the consumption of bacterial sources is low, inoculating efficiency is high, operability is high, pathogenesis is uniform, result stability is good, disease determination is highly sensitive, the method is applicable to the identification of physiological races of small samples and the identification of disease resistance in large-batch varieties, and the method serves for breeding novel disease-resistant varieties and reasonably laying out disease-resistant varieties.