The invention discloses a pulse generation system for an active and passive mixing locking die optical fiber laser, and belongs to the technical field of optical communication devices. The system has a main structure including an active locking die optical fiber laser resonance chamber which is composed of a pumping light source (1), a wavelength division multiplexer (2), a first photo-coupler (3), a polarizing controller (4) and the like, as well as a passive locking die optical fiber laser system which is composed of a dispersion compensating fiber (22), black phosphorus saturated absorber (21) and the like and a pulse optimizing system which is composed of two automatic feedback control rings. By adopting the active and passive mixing locking die technology, a part of output laser is received by a photodetector, an adjustable optical fiber delay line is controlled by utilizing feedback of a singlechip to realize optimization of output pulse of the whole system, so that stable ultra-short high-speed optical pulse of the whole system is produced, the operation is simple and accurate control can be achieved.