The invention discloses a rana nigromaculata seedling breeding method applicable to rice field culture. The method comprises the following steps of 1, tadpole seedling temporary rearing; 2, tadpole seedling feeding rearing; 3, tadpole seedling testing breeding; 4, froglet seedling breeding management, wherein froglet seedlings are fished out and placed into a breeding pond for performing breeding; 5, stocking, wherein the froglet seedlings are put into a rice field. The method has the advantages that by breeding rana nigromaculata tadpoles and young froglet seedlings, temporary rearing is performed in a temporary rearing tank, congenitally deficient diseased and disabled froglets are removed, then, PH value regulation is performed in a culture pond, the adaption degree of rana nigromaculata seedlings in the rice field is further improved, the survival rate and the diseases resistance of rana nigromaculata in the rice field are improved, the survival rate of rana nigromaculata in the rice field is increased, and the meat quality of rana nigromaculata is improved.