The invention provides a simulation signal monitoring system including an analog-to-digital converter, a register set, and a host. The monitoring system further comprises a multiplexer and an analog-to-digital conversion controller, the multiplexer provides more than two monitoring channels and enables the monitored analog signals to be selectively transmitted to the analog-to-digital converter. The simulation signal monitoring system and method are advantageous in that 1. the monitoring system can simultaneously monitor, sample and perform analog-to-digital conversion on various analog signals, so that the host can simultaneously capture the change of the analog signals in a short time; and 2. the analog-to-digital converter controller can reduce the load on the host and dynamically configure the analog signals to be monitored, and the host can read the analog channel data through the analog-to-digital conversion result register as needed.