The invention discloses a method for extracting chloroplast DNA of strip-leaf plants. The method comprises the following steps that A, the protoplasts of leaves are acquired by means of making mechanical injuries on the leaves and enzymolysis treatment in combination, wherein the acquired protoplasts contain chloroplasts, mitochondria and nuclei; B, impurities in the acquired products from step A are removed by differential centrifugation treatment to conduct crude extraction of the chloroplasts; C, the impurities in the acquired products from step B are removed by density gradient centrifugation to conduct purification of the chloroplasts; D, extraction is conducted on the acquired products from step C by means of a Tiangen rapid biochemical plant genome DNA extraction system to obtain high-purity chloroplast DNA. With the method for extracting the chloroplast DNA of the strip-leaf plants, high-purity and high-quality extraction can be conducted on the chloroplast DNA of the strip-leaf plants.