The invention discloses a method for testing a low-speed wireless network MAC protocol server. The method comprises the following steps: deploying an auxiliary testing gateway in a real scene, dividing wireless networks covered by the auxiliary testing gateway into a plurality of areas according to signal intensities, deploying a synchronous node in each of the areas, and networking the synchronous nodes to obtain short addresses; and simulating a node server to generate simulation nodes in batches and dividing the simulation nodes into a plurality of groups, wherein one group of the simulation nodes are corresponding to the short address of one synchronous node, so as to be synchronous with working parameters of the synchronous node. According to the method and the system for testing functionality, stability and throughput of the low-speed wireless network MAC protocol server under a high load provided by the invention, by simulating the node server to generate the simulation nodes in batches, and synchronizing the simulation nodes via the working parameters of a small amount of the synchronous nodes, the beneficial effects that the testing cost and the testing complexity are reduced and the testing cycle is shortened are achieved.