The invention discloses a high-accuracy temperature control method for a Sterling refrigerator. The high-accuracy temperature control method comprises the following steps of step I, obtaining a temperature signal of a temperature sensor arranged on a cold head of the refrigerator by a three-wire system Wheatstone bridge type temperature measuring circuit, after an output signal of the temperature sensor is processed by a differential amplifier circuit, enabling the processed output signal to enter a single-chip microcomputer, and performing calculation; step II, generating the actual temperature of the cold head of the refrigerator by sequentially adopting the transformation between a singular point eliminating algorithm and medium value filtering and step III, comparing the actual temperature with the set temperature, and adjusting and controlling the output current of a refrigerator power driving circuit by utilizing a parameter self-turning fuzzy PID algorithm so as to realize the high-accuracy temperature measurement and stable temperature control of the refrigerator. According to the high-accuracy temperature control method for Sterling refrigerator disclosed by the invention, the actual temperature of the cold head of the refrigerator is generated through the transformation between the medium value filtering and the singular point eliminating algorithm, the actual temperature and the set temperature are compared, and the output current of the refrigerator power driving circuit is adjusted and controlled through the parameter self-turning fuzzy PID control algorithm, so that the stable temperature control of the refrigerator is realized.