The invention discloses a dog food. The dog food consists of the following components in parts by mass: 6-15 parts of pork, 8-15 parts of beef, 7-13 parts of konjak, 10-15 parts of corn, 30-40 parts of wheat, 5-15 parts of millet, 10-12 parts of soybean flour, 5-8 parts of fish meal, 8-15 parts of bone meal, 0.3-0.5 part of milk powder, 0.1-0.2 part of eggs, 6-8 parts of watermelon peel, 10-12 parts of white gourds, 0.1-0.2 part of lotus leaves, 3-5 parts of Chinese jujubes and 6-11 parts of carrots. A preparation method of the dog food comprises the following steps of (1) cooking the pork and the beef; (2) mixing the konjak with the corn, the wheat, the millet and the soybean flour so as to obtain gelatinized liquid; (3) performing pulping on the watermelon peel, the white gourds, the lotus leaves, the Chinese jujubes and the carrots with a pulping machine to obtain pulp for standby application; (4) adding the gelatinized liquid obtained in the step (2), the pulp obtained in the step (3), the fish meal, the bone meal and the milk powder and the eggs in the cooked pork and the cooked beef obtained in the step (1) so as to obtain a primary product of the dog food; and (5) preparing finished products: performing puffing to obtain bar-shaped products, performing cooling to room temperature, performing sterilization and performing packing. The dog food disclosed by the invention is rich in nutrition, and preservation is facilitated.