The invention discloses a closed bipolar electrode array-based analysis detection device. The analysis detection device is characterized in that the analysis and detection device comprises a signal cell (10) array and a detection cell (11) array which are symmetrically arranged on a bearing layer, ever signal cell (10) comprises a signal acquisition terminal (5) of a bipolar electrode and a driving electrode A (7), every detection cell (11) comprises a detection terminal (6) of the bipolar electrode, a driving electrode B (8) and a reference electrode (9), the signal acquisition terminal (5) of the bipolar electrode and the detection terminal (6) are connected through a conductive layer (2) on the bearing layer, and the driving electrode A (7) and the driving the electrode (B) are connected with an external power source through the conductive layer (2) on the bearing layer. The detection device effectively solves the problem of the influence of potential drift of the bipolar electrode on the analysis detection precision during the analysis detection of present closed bipolar electrode.