The invention provides a method for microbial remediation of mine tailings and mine tailing wastewater via Rhizopus metabolites and microzymes. A set of a remediation system is built by adding the Rhizopus fermentation products of deciduous leaves of plants and the microzymes into mine tailings and mine tailing wastewater. Addition of the Rhizopus fermentation products the microzymes realizes inhibition of oxidation bacteria existing in the mine tailings and leading to dissolving-out of heavy metals, and the microzymes can stably exist for a long time; physical and chemical indexes show that addition of the Rhizopus metabolites and the microzymes leads to increase of pH value and reduction in redox potential and dissolved ions; thus, the microbial remediation method exerts remediation effect on a tailings pond and mine tailing wastewater.