The invention relates to a method for determining malachite green, quinolone and sulfonamide drugs in aquatic products. The method comprises the following steps: step 1, preparing samples; step 2, extracting to-be-detected samples to obtain to-be-processed samples; step 3, determining chromatographic conditions; step 4, determining mass spectrum conditions; step 5, drawing a standard working curve; step 6, determining sensitivity, accuracy and precision. With adoption of the method, malachite green, leucomalachite green, quinolone and sulfonamide drugs in an extracted and concentrated aquatic product substrate are determined simultaneously for the first time, the problems that time is consumed and detection cost is high due to traditional respective extraction are solved, ion response value is increased, sensitivity is enhanced, detection limit is low, the effects of being qualitative, quantitative, accurate, fast, efficient and sensitive are realized truly, and the method can be taken as a reliable detection means for the three drugs and is applicable to extraction and concentration of a batch of samples.