The invention discloses a preparation method of an antifouling and fingerprint-proof photocured fluorine-containing resin coating film. Fluorine-containing hydroxyl resin and polyfunctional isocyanate react firstly, hydorxy-olefinic compounds are added for end capping, a polyfunctional fluorine-containing alkenyl resin intermediate is prepared, a coating is prepared from the intermediate, 2,6-functional acrylate, alkenyl-modified nano-reinforcing components, a photoinitiator and the like through compounding and is applied to the surface of a base material such as glass, metal or the like, levelling, surface drying, baking and photocuring are performed again, and the antifouling and fingerprint-proof transparent coating film can be obtained.