The invention relates to an anti-rotation lifting cylinder. The anti-rotation lifting cylinder comprises a cylinder barrel, a piston assembly, a piston rod, a front cover and a back cover, and further comprises a mounting seat, an end cover, a connecting screw and an air guide tube, wherein the front cover and the back cover are separately arranged at the two ends of the cylinder barrel; the piston rod is arranged in the cylinder barrel; the front end of the piston rod is fixedly connected with the piston assembly; the piston rod and the back cover are in anti-rotation fit; the back cover, the mounting seat and the end cover are sequentially fixed; the connecting screw is arranged in the mounting seat; the front end of the connecting screw is fixedly connected with the back end of the piston rod; the back end of the connecting screw stretches into the end cover; the piston rod and the connecting screw are hollow; the air guide tube is arranged in the piston rod and the connecting screw in an interpenetrating manner; the front end of the air guide tube is fixedly connected with the piston assembly; and the back end of the air guide tube stretches into the end cover. According to the anti-rotation lifting cylinder, the structure is simple and reasonable, stress is balanced, control and locating precision is high, the piston rod can be prevented from being broken, and the service life of the cylinder is prolonged.