The invention relates to an aquaculture water chamber used for a movable aquaculture factory ship. The aquaculture water chamber comprises a fish farming water chamber body, and the fish farming water chamber body is internally provided with water obtaining traps which pump deep and far sea water to convey the sea water into the fish farming water chamber body; a center line bulkhead is arranged in the middle of the fish farming water chamber body and divides the fish farming water chamber body into two independent chambers including the left chamber and the right chamber; overflow pipes are arranged at the bottom of the fish farming water chamber body and provided with butterfly valves, and the overflow pipes are connected to the positions above designed drafts of sides along the side walls of the fish farming water chamber body; by opening the control butterfly valves of the overflow pipes, water continuously overflows from the bottom of the water chamber to the outside of ship boards to achieve water flow exchange. The water chamber can meet the requirements ofwater flow exchange and dirt discharge of large chamber aquaculture.