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发明公开
CN106868048A 抵抗饮食诱导肥胖的CTRP6干扰方法 无效 - 驳回

抵抗饮食诱导肥胖的CTRP6干扰方法
摘要：
本发明公开了一种抵抗饮食诱导肥胖的CTRP6干扰方法，包括以下步骤：(1)重组慢病毒干扰载体的构建；(2)CTRP6干扰慢病毒的包装；(3)CTRP6干扰慢病毒的滴度测定；(4)pLentiH1‑CTRP6‑shRNA慢病毒侵染小鼠脂肪细胞效果；(5)pLentiH1‑CTRP6‑shRNA对小鼠组织中CTRP6表达的影响。本发明所述的抵抗饮食诱导肥胖的CTRP6干扰方法通过pLentiH1‑CTRP6‑shRNA慢病毒能显著降低脂肪组织中CTRP6 mRNA的表达水平，且脂肪组织中CTRP6的蛋白水平也明显下降，实现对小鼠组织和血清中CTRP6的干扰作用。
摘要（英）：

The invention discloses a CTRP6 interfering method for resisting diet-induced obesity. The method comprises the following steps: (1) building a recombinant lentivirus interference vector; (2) packaging CTRP6 interference lentiviruses; (3) measuring the titer of the CTRP6 interference lentiviruses; (4) infecting mice adipose cells through pLentiH1-CTRP6-shRNA lentiviruses; (5) influencing the CTRP6 expression in mice tissues by pLentiH1-CTRP6-shRNA. By adopting the CTRP6 interfering method for resisting the diet-induced obesity, the expression level of CTRP6 mRNA (messenger Ribonucleic Acid) in fat tissues can be lowered remarkably through the pLentiH1-CTRP6-shRNA lentiviruses, the protein level of CTRP6 in the fat tissues is lowered remarkably, and interference on the CTRP6 in the mice tissues and serum is realized.

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