The invention discloses a manual perforating machine with the positioning function. The manual perforating machine comprises a base, a supporting rod, a machine head and a clamping mechanism. The clamping mechanism comprises a stand column, and the axial direction of the stand column is perpendicular to the plate surface of the base. One axial end of the stand column is fixed to the base, and the other axial end of the stand column is provided with a cross arm. The axial direction of the cross arm is perpendicular to that of the stand column. One axial end of the cross arm is connected with the stand column, and the other axial end of the cross arm is provided with a fixing tube. The axis line of the fixing tube is perpendicular to the plate surface of the base, and the inner wall of the fixing tube is provided with an internal thread in the axial direction. The manual perforating machine further comprises an adjusting rod. The axial outer wall of the adjusting rod is provided with an external thread matched with the internal thread. One end of the adjusting rod is screwed into the fixing tube successively and penetrates through the cross arm to extend in the direction close to the base. A pressing plate is arranged at the end of the direction, close to the base, of the adjusting rod. Users can fixedly clamp to-be-perforated objects of different thicknesses through the clamping structure, and fast and accurate perforation operation can be conducted by the users conveniently.