The invention belongs to the technical field of tobacco processing, and particularly relates to a treatment method for lowering the cut stem crotonaldehyde release amount. The method comprises the steps of uniformly spraying a yeast aqueous solution into cut stems for fermentation treatment, drying the cut stems and the like for taking the cut stems as application objects. According to the method, by means of co-use of the fermentation process, the amino acid adding process and the like, on one hand, the crotonaldehyde precursor content is lowered, and on the other hand, release of crotonaldehyde is inhibited, so that the technical objective of lowering the crotonaldehyde release amount is achieved. Furthermore, by adopting a biological enzyme and adding the amino acid component, foreign flavor of the cut stems is effectively removed, Maillard reaction products in smoke are increased, and the cigarette sensory quality is improved and stabilized. In a word, the treatment method has the advantages of being simple, easy to implement, low in cost, high in safety and the like, large-scale and industrial application is promoted, and important application value is achieved for improving the cigarette quality and expanding the cut stem application range.