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发明授权
CN106854649B 金黄色葡萄球菌肠毒素C2的核酸适配体C204及其筛选方法和应用 有权

金黄色葡萄球菌肠毒素C2的核酸适配体C204及其筛选方法和应用
摘要：
本发明涉及一种金黄色葡萄球菌肠毒素C2的核酸适配体C204及其筛选方法和应用，所述核酸适配体C204的序列为：AGGGCCGAGCTCACTTGTCTTAGTCCGACATGGTGCCTCCCTGCCCCAGAGCTCCGCCGGCACAATTGTGC；它的筛选方法为：基于核酸适配体的体外SELEX筛选技术，利用羧基磁珠作为固相介质，以金黄色葡萄球菌肠毒素C2为靶标，通过金黄色葡萄球菌肠毒素C2磁珠从ssDNA文库中筛选得到与金黄色葡萄球菌肠毒素C2特异性结合的核酸适配体；本发明的核酸适配体C204能高亲和力、高特异性地与金黄色葡萄球菌肠毒素C2结合。
摘要（英）：

The invention relates to an aptamer C204 of staphylococcal enterotoxin C2, a screening method and application thereof. The sequence of the aptamer C204 is: AGGGCCGAGCTCACTTGTCTTAGTCCGACATGGTGCCTCCCTGCCCCAGAGCTCCGCCGGCACAATTGTGC. The screening method includes: adopting an aptamer based in-vitro SELEX (systematic evolution of ligands by exponential enrichment) screening technology, utilizing carboxyl magnetic beads as the solid medium, taking staphylococcal enterotoxin C2 as the target, and screening the aptamer specifically bonded to staphylococcal enterotoxin C2 from an ssDNA library through the staphylococcal enterotoxin C2. The aptamer C204 provided by the invention can realize high affinity and high specificity binding with the staphylococcal enterotoxin C2.

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