The invention belongs to the technical field of dry storage of spent fuel of a nuclear power station, and particularly relates to a grabbing and loading device of a spent fuel basket. The grabbing and loading device of the spent fuel basket is used in a way that the spent fuel basket is grabbed and lifted to a proper position, and is conveyed to a destination by a corresponding conveying tools, then the spent fuel basket is descended to the proper position by the grabbing and loading device of the spent fuel basket, and a grabbing part of the grabbing and loading device is separated from the spent fuel basket. The grabbing and loading device of the spent fuel basket is characterized by comprising an electric hoist chain, an air cylinder piston, a transmission rod, steel balls, a microswitch and a casing, wherein the casing is lifted at the lower end of the electric hoist chain; the air cylinder piston, the transmission rod, the steel balls and the microswitch are positioned in the casing; the transmission rod is fixedly arranged at the lower end of the air cylinder piston; the microswitch is positioned at the outer side of the transmission rod; the steel balls are positioned at the outer side of the transmission rod, and are also positioned under the microswitch. The grabbing and loading device has the advantage that the spent fuel basket can be grabbed, released and recycled, so that the accidental injury risk to workers and the damage risk to equipment are prohibited.