The invention relates to a glue plug hardness measuring device. A positioning mechanism, a pneumatic detection mechanism, an electric air pressure generation device, an air pressure starting pedal and a height limiting device are arranged on a workbench separately; the positioning mechanism is used for positioning a glue plug part; the pneumatic detection mechanism is arranged above the positioning mechanism and is used for detecting the glue plug part in the positioning mechanism; the electric air pressure generation device is arranged on one side of the pneumatic detection mechanism and is used for providing air pressure for the pneumatic detection mechanism; the air pressure starting pedal is used for starting power supply for the electric air pressure generation device; the height limiting device is arranged on one side of the positioned mechanism and positioned below the pneumatic detection mechanism, and is used for limiting the descending height of the pneumatic detection mechanism; the air supply end of the electric air pressure generation device is connected with the air inlet end of the pneumatic detection mechanism through a vacuum pipe; and the air pressure starting pedal is connected with the power supply input end of the electric air pressure generation device through a pedal wire. The glue plug hardness measuring device guarantees the consistency of direction, force and stress surface of the pressure borne by the glue plug part in the measuring process, improves measurement stability and accuracy, and realizes repeatability and reproducibility requirements of measuring data of the glue plug part.