The invention relates to the technical field of home textile and discloses a preparation method of a bamboo fiber bacteriostatic towel. A base material is prepared from raw materials including bamboo fibers, ramie fibers and polyester fibers, loops are prepared from raw materials including bamboo fibers, cotton fibers, modal fibers and down, the ramie fibers, the polyester fibers, the cotton fibers and the modal fibers are placed in a bacteriostatic liquid to be soaked and subjected to microwave radiation, then the base material is prepared by blending the bamboo fibers, the ramie fibers and the polyester fibers, the bacteriostatic liquid is uniformly applied to two sides of the base material and subjected to high/low-temperature alternating treatment, loop down is obtained by blending the bamboo fibers, the cotton fibers, the modal fibers and the down, the bamboo fiber bacteriostatic towel is prepared by spinning the base material and the loop down, the towel has a lasting bacteriostatic effect by the aid of the selected bamboo fibers and the ramie fibers, the bacteriostatic liquid is subjected to microwave irradiation to be immersed in raw material fibers, and a bacteriostatic layer can adhere lastingly under the high/low-temperature alternation action, so that the towel has three-layer bacteriostatic protection.