The invention discloses a method and device used for determining a picking route. The method comprises the steps that orders cut off in a preset time period are obtained; an aggregation order is built according to storage areas of product types contained in the orders; a warehouse map is obtained, wherein the warehouse map comprises coordinates of following nodes including the picking table node, the traffic crossing node, the alley crossing node and the virtual storage location node; based on the aggregation order and the warehouse map, the picking route is determined. By means of the method, the aggregation order is built according to the storage areas of the product types, and when conducting order picking according to the aggregation order, the required picking route is short, so that the picking cost of the picker is reduced and the picking efficiency of the picker is improved; the picking route is determined based on the aggregation order and the warehouse map so that it can be determined that the route from which storage locations of products in the aggregation order are traversed is shortest, accordingly the total picking route is short, and the total picking efficiency of the warehouse is improved.