The invention provides a safety locking device of a partition door of a subway tunnel. The device comprises a base, a lifting table arranged on the base and the partition door arranged on one side of the lifting table, wherein a limiting stop is arranged at the position, close to the partition door, of the lifting table; the lifting table is provided with an elastic assembly perpendicular to the bottom face of the lifting table; one end of the elastic assembly is fixed to the limiting stop; the other end of the elastic assembly is fixed to the bottom of the lifting table; a locking mechanism is arranged on one side of the lifting table; the limiting stop bounces and resets after the partition door is rotated and opened to pass through the limiting stop; and the locking mechanism abuts against and is supported at the bottom of the partition door after the partition door is rotated and opened. The safety locking device achieves the effects that the partition door is firm and reliable, operation is more convenient and saves time, and safety and reliability of the partition door are improved.