The invention relates to the technical field of biology, in particular to an effectively passage method for human diploid cell microcarrier culture in a bioreactor microcarrier scale-up culture process. According to the method, a microcarrier is utilized to culture a human diploid cell seed, a blank microcarrier is prebalanced in a seed culture system, a seed cell is digested by trypsin digestive juice containing sodium citrate, the seed cell is moderately stirred and cultured discontinuously for 3 to 6 hours in a seed reactor, and then the seed cell is transferred to an amplification reactor to be amplified and cultured to obtain effective passage in the human diploid cell microcarrier scale-up culture process. The effective passage method disclosed by the invention has effects of improving vigor, adherence rate and adherence evenness of cells in a passage process, obviously improving passage efficiency and solving the problem of difficulty in scale-up culture of the human diploid cells on the bioreactor and the microcarrier.