The invention discloses an artificial tissue globular cluster construction device and method. The device comprises a slide glass, a polydimethylsiloxane (PDMS) packing sheet, an insertion piece and a pressing plate, wherein a cavity is formed in the PDMS sheet; endothelial cells are induced to encircle circular gel containing tissue cells in the cavity by virtue of a biological factor so as to form a tight endothelial thin layer, and the artificial tissue globular cluster coated by the functional endothelial layer is constructed. The globular cluster produced in the invention can simulate reactions of corresponding tissues on physicochemical or biochemical stimulation under the protection of the vascular endothelial layer, and when the globular cluster is applied to drug screening, pathological study and therapy detection, accurate results can be obtained. The globular cluster constructed on the PDMS sheet can respectively integrate a physicochemical/biochemical stimulation control module and a detection module at the inlet and outlet, so that corresponding stimulation-detection model construction on a chip is realized; meanwhile, the slide glass can be removed after the endothelial layer is formed, and the globular cluster is taken out to be used for subsequent complicated model construction or detection.