The invention discloses a high-calcium scheelite flotation collector and a preparation method and an application method thereof and belongs to the technical field of solid mineral resource sorting. The preparation method comprises the following steps of: by taking foots, hydroxylamine hydrochloride, sodium hydroxide, sulfuric acid and the like in a coconut oil refining process as raw materials, adding the treated foots in a certain molar ratio into a hydroxylamine hydrochloride aqueous solution at a certain temperature; then dropwise adding a sodium hydroxide aqueous solution with a certain concentration, and performing acidification after the dropwise adding reaction is finished; dehydrating and washing the acidified product to obtain coconut alkylhydroxyoxime acid; and then compounding the coconut alkylhydroxyoxime acid and soy acid in certain percentages by mass to obtain a mixture, and then saponifying the mixture to obtain the high-calcium scheelite flotation collector. The collector prepared by the preparation method disclosed by the invention is stable in property, good in floating index, stable in foam, high in adaptability, low in cost and non-toxic, and meanwhile, can efficiently separate scheelite from calcium-containing minerals in the high-calcium scheelite.