The invention provides a compound imidacloprid drop, and a preparation method and application thereof. The compound imidacloprid drop has a good insecticidal effect by complexly using components having the insecticidal effect such as imidacloprid and permethrin, and can be used for killing animal epizoa. Meanwhile, in the compound imidacloprid drop, propylene glycol, laurocapram, eucalyptus oil and moringa seed oil are taken as a transdermal agent, wherein the moringa seed oil and the eucalyptus oil are natural plant essential oil, are aromatic, and can cover foreign flavors in the drop and improve the sense of a user. Further, usage of a composite transdermal agent can effectively improve the permeation of the compound imidacloprid drop. Meanwhile, in the preparation method, the components serving as raw materials are mixed to prepare the corresponding drop. The preparation method is simple, the efficiency is high, and the insecticidal effect of the prepared drop is good.