The invention discloses an ultrasonically-guided mammary gland puncture device which comprises a large ring, a small ring, an ultrasonic probe and a vacuum air suction device. An annular first cavity is arranged inside the large ring in a manner of surrounding the same by a round, more than one groove is formed in the undersurface of the large ring in a manner of surrounding the large ring by a round, a large sucker is arranged in each groove, an air suction end of each large sucker is communicated with the first cavity through a first air suction tube, a first air tube is fixedly mounted on the outer ring face of the large ring, one end of the first air tube is fixedly connected with the vacuum air suction device through a hose, an annular sliding groove is formed in the surface of the large ring in a manner of surrounding the large ring by a round, a slider is slidably mounted in the sliding groove, and one end of the slider penetrates an opening of the sliding groove to extend to the outside and is fixedly provided with an arc-shaped rod. The ultrasonically-guided mammary gland puncture device is simple in structure and can quickly fix a portion needing puncture, so that moving of a puncture portion is avoided; positioning can be effectively realized through a puncture barrel, so that puncture speed is increased.