The invention relates to a tissue culture method using a bacteria inhibiting culture medium to culture panicum virgatum. The tissue culture method comprises the following steps of disinfection of a culture container, preparation of the culture medium, preparation of sterile water, inducing culture, proliferation of healing tissues, differentiation culture, rooting culture and bottle seedling transplanting. The panicum virgatum bacteria-inhibiting tissue culture method has the advantages that the high-temperature and high-pressure sterilization of culture container and culture medium is not needed, the workload and energy consumption are decreased, the panicum virgatum tissue culture technical step is simplified, and the tissue culture cost of the panicum virgatum is reduced; the operation is simple, the method can be used after different culture medium formulas are used, the practicality is strong, and the popularizing property is good; compared with conventional panicum virgatum tissue culture method, the cost is reduced by more than 10%.