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发明公开
CN106701792A 人工合成的对鳞翅目害虫高毒力杀虫基因及应用 有权

人工合成的对鳞翅目害虫高毒力杀虫基因及应用
摘要：
本发明为“人工合成的对鳞翅目害虫高毒力杀虫基因及应用”，属生物防治技术领域。本发明提供的对鳞翅目害虫具有高毒力的mcry2Ah基因、mcry2Ah‑vp基因和mcry2Ah‑sp基因的人工优化、改造并合成的新的DNA序列(见SEQ ID NO2、SEQ ID NO3和SEQ ID NO4)，利用植物偏好的密码子序列，可以提高mcry2Ah基因、mcry2Ah‑vp基因和mcry2Ah‑sp基因在转基因植物中的表达量和稳定表达的特性，mcry2Ah‑sp表现出对抗性鳞翅目害虫的抗虫性高于其他改造基因，可以利用该基因序列转化植物防治害虫和延缓害虫抗性产生。
摘要（英）：

The invention relates to an artificially synthesized insecticidal gene with high toxicity on lepidoptera pests and application, and belongs to the technical field of biological control. The artificially synthesized insecticidal gene has the advantages that a mcry2Ah gene, a mcry2Ah-vp gene and a mcry2Ah-sp gene with high toxicity on the lepidoptera pests are subject to artificial optimizing and modification, and are synthesized into a new DNA (deoxyribonucleic acid) sequence (shown in SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4), a codon subsequence preferable by plants is utilized, and the expression quantity and stable expression characteristic of the mcry2Ah gene, mcry2Ah-vp gene and mcry2Ah-sp gene in transgenic plants are improved; compared with other modified genes, the mcry2Ah-sp gene has higher pest resistance on the resistance lepidoptera pests, and the gene sequence can be used for transforming the plant to prevent and control pests and delay the forming of pest resistance.

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