The invention discloses a silane coupling agent containing a vinyl ether chain segment, and a preparation method and an application thereof. The silane coupling agent is a novel silane coupling agent. The silane coupling agent is acquired through the catalytic reaction of the traditional coupling agent with a formula (IV) structure and fatty alcohol-polyoxyethylene ether or alicyclic alcohol polyoxyethylene ether. The application of the silane coupling agent disclosed by the invention in preparing a high-performance tread rubber by in situ modifying white carbon black can effectively reduce the VOC discharge in the size mixing process and in the use process of rubber products. The silane coupling agent disclosed by the invention also can be used for modifying the liquid phase of the white carbon black; the wet-process mixing can be realized; the problems of single method for modifying the white carbon black with the traditional silane coupling agent, large energy consumption in the processing process and serious pollution can be effectively solved; and the traditional coupling agent with the formula (IV) structure and the fatty alcohol-polyoxyethylene ether in low cost are taken as the synthesis raw materials, so that the use cost of the coupling agent is lowered.