The invention discloses a tabebuia chrysantha tissue culture and rapid propagation method. The tabebuia chrysantha tissue culture and rapid propagation method comprises the steps of treatment and disinfection of an explants, acquisition of aseptic seedlings, differentiation and proliferation of aseptic buds and culture and transplanting of rooting seedlings. A propagation method and a culture medium formula of the invention are used for carrying out tabebuia chrysantha tissue culture, the annual growth coefficient of the aseptic buds reaches 3.012, and the rooting rate reaches 97 percent. The tabebuia chrysantha tissue culture and rapid propagation method is provided by using tabebuia chrysantha seeds as the explants, an effective path is provided for seedling breeding of tabebuia chrysantha, and large-scale production requirements of the seedling breeding are met.