The invention belongs to the technical field of virus elimination of plants, and particularly relates to a virus-elimination method of a plant tissue cultured virus-free explant. The method comprises the following steps: selecting plant mature tissues, performing sterilizing treatment on the selected plant mature tissues, performing primary culture on the sterilized tissues in a primary culture medium, performing propagation culture based on the primary culture, performing virus detection based on the propagation culture, sorting secondary seedlings of which the viruses are negative, extending reproductive output, then performing rooting culture until favorable root systems grow, and then performing domestication and transplanting. Mature tissues are used as explants, so that the tissue culture virus elimination operation is simple, the differentiation rate and the survival rate are greatly increased, and the virus-elimination success rate is high.