The invention discloses an anti-static conductive fiber material preparation method, and belongs to the technical field of conductive fiber preparation. Bean hawk moth larvae rich in protein are manually treated and then subjected to enzymolysis to prepare polypeptide chelating agents, polyacrylonitrile fibers are carbonized, then mixed, fermented and micro-corroded with aspergillus and grape skins, blended with metallic salt solution, fermented and reduced to obtain conductive fibers. The preparation method has the advantages that preparation steps are simple, conductive fiber packing does not fall off from surfaces of the fibers and is uniformly distributed, the prepared conductive fibers are good in conductivity and long in conductive durability and have excellent anti-static effects, and conductive durability is improved by 29.5-31.2%.